Synthesis and Cytotoxic Activity of Some 1,2,4-Triazoline and 2,5-Disubstituted Derivatives

Abstract

In this search, a series of 5-[4-(4-fluorobenzoylamino) phenyl]-2-substitutedamino- 1,3,4-thiadiazole (2a-g) and 5-[4-(4-fluorobenzoylamino) phenyl]-4-substituted-2,4-dihydro- 3H-1,2,4-triazo-le-3-thione (3a-f) derivatives were synthesized and characteristed by elemental analysis, UV-visible, IR, H-1-NMR, MS spectral data and X-ray crystallograpy (3b). Cytotoxic activity of six prototype compounds (2a, 2d, 2e, 3a, 3d and 3e) were evaluated by using HeLa (ATCC CCL-2) and normal cell lines according to procedures of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] Assay [Cell Proliferation Kit I (MTT) Roche-Germany].