Preventive and Protective Effects of Turkish Propolis on H2O2-Induced DNA Damage in Foreskin Fibroblast Cell Lines

Abstract

The aim of the present study was to evaluate the potential of Turkish propolis extracts if they prevent or protect foreskin fibroblast cells against hydrogen peroxide (H <inf>2</inf>O <inf>2</inf>)-induced oxidative DNA damage. Hydrogen peroxide (40 μM) was used as an inducer of oxidative DNA damage. The damage of DNA was evaluated by using the alkaline single cell gel electrophoresis (comet) assay. Turkish propolis extracts at concentrations of 25, 50, 75 and 100 μg/ml were prepared by ethanol. Anti-genotoxicity was assessed before, simultaneously, and after treatment of propolis extract (50 μg/ml) with H <inf>2</inf>O <inf>2</inf>. The results showed a significant decrease in H <inf>2</inf>O <inf>2</inf>-induced DNA damage in cultures treated with propolis extract. The antioxidant activity of phenolic components found in propolis may contribute to reduce the DNA damage induced by H <inf>2</inf>O <inf>2</inf>. Our findings confirmed the chemopreventive activity of propolis and showed that this effect may occur under different mechanisms. © 2011 Akademiai Kiado, Budapest.