Association of IL6-174G > C and IL10-1082A > G Genetic Polymorphisms with Cervical Insufficiency in the Turkish Population

Abstract

Background A recurring, passive, and painless dilatation of the cervix in the second trimester is known as cervical insufficiency. Although the exact cause is unknown, a subclinical intra-amniotic infection may be linked to it. Downregulation of anti-inflammatory cytokines like interleukin 10 (IL10), in contrast to upregulation of pro-inflammatory cytokines like interleukin 6 (IL6), is linked to pregnancy-related preterm birth. This research aims to investigate the potential correlation between the polymorphisms -174G>C (rs1800795) in IL6 and -1082A>G (rs1800896) in IL10 and cervical insufficiency. Methods DNA samples from 93 patients with cervical insufficiency and 103 healthy controls were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. Statistical analysis was performed using the chi-square test. Results According to composite genotyping analysis, the CC/AA (IL6 -174G>C/IL10 -1082A>G) composite genotype revealed a significant difference between patients and controls (p = 0.049). The number of abortions and CC/AG (IL6 -174G>C/IL10 -1082A>G) composite genotype was also associated with each other (p = 0.006). However, any statistically significant difference wasn't found between patients and controls in terms of allele and genotype distributions of IL6 -174G>C and IL10 -1082A>G polymorphisms separately (p > 0.05). Conclusions Although we did not find an association between IL6 -174G>C (rs1800795) and IL10 -1082A>G (rs1800896) polymorphisms and cervical insufficiency individually, it seems that these polymorphisms together may predispose to the disease and its severity.