Characterization and Comparative Investigation of in Vitro Bioactivities for Lactuca Anatolica Root Aqueous Extract and Their Green-Chemical Synthesized Nanoparticles; Molecular Docking Studies

Abstract

The current study synthesized silver nanoparticle (AgNP) biosynthesis and sustainable biochemical approaches, molecular docking studies using endemic and medicinal L. anatolica root aqueous extract. AgNPs were characterized in detail by TEM, XRD, and spectroscopic techniques such as FT-IR and UV-vis. The bioactivity of AgNPs was investigated with antioxidant, anti-inflammatory, photocatalytic, antibacterial, enzyme inhibition, and DNA protection tests. The AgNP formation was verified by UV-vis examination at 440 nm and the XRD, which showed a clearly defined crystalline organization. The TEM picture revealed uniformly distributed with a spherical shape and an average diameter of 17.5 nm. The synthesized AgNP exhibited PMRA (A(0.5), 0.10 mu g/mL), RPA (A(0.5), 1.17 mu g/mL), LPI (IC50, 1.10 mu g/mL), DPPH center dot (IC50, 2.61 mu g/mL), and ABTS(center dot+) (IC50-6.21 mu g/mL) scavenging activities higher activity than the aqueous extract and the standards. Photocatalytic activity of AgNP exhibited significant methylene blue dye degradation efficiency when exposed to visible light for 5 h. AgNP displayed efficient inhibition of BSA denaturation with 57.58% at 125 mu g/mL. Urease, BChE, and alpha-amylase inhibition activities of AgNP were more effective than aqueous extract and drugs. AgNP had excellent antibacterial activities, with an 8.5 and 9.0 mm inhibition zone against Bacillus cereus and Enterococcus faecalis. In silico studies chlorogenic acid-AgNP interaction product, the antibacterial activity of AgNPs was consistent with their in vitro effectiveness, as high molecular docking scores between - 203 and - 238. It was observed that AgNP protected DNA against oxidative damage more than quercetin. This is the first report confirming the strong antioxidation, antimicrobial, enzyme regulatory, anti-inflammatory, photocatalytic, and DNA protector of aqueous extract and AgNP.