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Serologic Detection of Antibodies against Fasciola Hepatica in Sheep in the Middle Black Sea Region of Turkey

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dc.authorscopusid18433560100
dc.authorscopusid6504499889
dc.authorscopusid23484185000
dc.authorscopusid17342481800
dc.authorscopusid36125096200
dc.authorscopusid6603241369
dc.contributor.authorAçıcı, M.
dc.contributor.authorBüyüktanir Yaş, O.
dc.contributor.authorBölükbaş, C.S.
dc.contributor.authorPekmezci, Gökmen Zafer
dc.contributor.authorGürler, A.T.
dc.contributor.authorUmur, S.
dc.date.accessioned2020-06-21T13:19:19Z
dc.date.available2020-06-21T13:19:19Z
dc.date.issued2017
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Açıcı] Mustafa, Department of Parasitology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Büyüktanir Yaş] Özlem, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Bölükbaş] Cenk Soner, Department of Parasitology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Pekmezci] Gökmen Zafer, Preclinical Sciences, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Gürler] Ali Tümay, Department of Parasitology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Umur] S., Department of Parasitology, Ondokuz Mayis Üniversitesi, Samsun, Turkeyen_US
dc.description.abstractBackground/Purpose The aim of the present study was to estimate the prevalence of Fasciola hepatica infection in sheep in the Black Sea region of Turkey. Methods Samples from 213 sheep were collected randomly in Samsun, Tokat, and Sinop from September 2005 to January 2007 and tested by indirect enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using F. hepatica excretory-secretory (E/S) antigens. Results The distribution of ELISA-positive samples for F. hepatica infections out of a total of 213 sheep serum samples was 23/71 (32.4%), 15/59 (25.4%), and 29/83 (34.9%) in Samsun, Sinop, and Tokat, respectively. The immunodominant proteins were determined by Western blot analysis using molecular weight markers of 14 kDa, 20 kDa, 24 kDa, 27 kDa, 33 kDa, 45 kDa, and 66 kDa and extracted from sera of sheep that were positive for Fasciola spp. eggs and also hyperimmune sera from rabbits immunized with E/S antigens. Conclusion The ELISA-positive results were confirmed by Western blot analysis. As a result, seroprevalence of F. hepatica infection was found in 31.4% of sheep from the Karayaka breed in the Middle Black sea region of Turkey. © 2015en_US
dc.identifier.doi10.1016/j.jmii.2015.06.005
dc.identifier.endpage381en_US
dc.identifier.issn1684-1182
dc.identifier.issn1995-9133
dc.identifier.issue3en_US
dc.identifier.pmid26303043
dc.identifier.scopus2-s2.0-85023175193
dc.identifier.scopusqualityQ1
dc.identifier.startpage377en_US
dc.identifier.urihttps://doi.org/10.1016/j.jmii.2015.06.005
dc.identifier.volume50en_US
dc.identifier.wosWOS:000406110600016
dc.identifier.wosqualityQ2
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofJournal of Microbiology Immunology and Infectionen_US
dc.relation.journalJournal of Microbiology Immunology and Infectionen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectELISAen_US
dc.subjectFasciolosisen_US
dc.subjectSheepen_US
dc.subjectTurkeyen_US
dc.titleSerologic Detection of Antibodies against Fasciola Hepatica in Sheep in the Middle Black Sea Region of Turkeyen_US
dc.typeArticleen_US
dspace.entity.typePublication

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