Streptomyces ovatisporus sp. nov., Isolated from Deep Marine Sediment

Abstract

The taxonomic position of a Gram-staining-positive strain, designated strain S4702T was isolated from a marine sediment collected from the southern Black Sea coast, Turkey, determined using a polyphasic approach. The isolate was found to have chemotaxonomic, morphological and phylogenetic properties consistent with its classification as representing a member of the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree. S4702T was found to be most closely related to the type strains of Streptomyces marinus (DSM 41968T; 97.8% sequence similarity) and Streptomyces abyssalis (YIM M 10400T; 97.6%). 16S rRNA gene sequence similarities with other members of the genus Streptomyces were lower than 97.5%. DNA–DNA relatedness of S4702T and the most closely related strain S. marinus DSM 41968T was 21.0%. The G+C content of the genomic DNA was 72.5 mol%. The cell wall of the strain contained L,L-diaminopimelic acid and the cell-wall sugars were glucose and ribose. The major cellular fatty acids were identified as anteiso-C<inf>15:0</inf>, iso-C<inf>16:0</inf>, anteiso-C<inf>17:0</inf> and iso-C<inf>15:0</inf>. The predominant menaquinone was MK-9(H<inf>8</inf>). The polar lipid profile of S4702T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. S4702T could be distinguished from its closest phylogenetic neighbours using a combination of chemotaxonomic, morphological and physiological properties. Consequently, it is proposed that S4702T represents a novel species of the genus Streptomyces, for which the name Streptomyces ovatisporus sp. nov. is proposed. The type strain is S4702T (DSM 42103T=KCTC 29206T=CGMCC 4.7357T). © 2016 IUMS.