Publication:
Differential Recovery of Bacterial and Archaeal 16S rRNA Genes from Ruminal Digesta in Response to Glycerol as Cryoprotectant

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dc.authorscopusid6602694996
dc.authorscopusid9846362800
dc.authorscopusid35727177000
dc.authorscopusid7401496291
dc.contributor.authorMcKain, N.
dc.contributor.authorGenç, B.
dc.contributor.authorSnelling, T.J.
dc.contributor.authorWallace, R.J.
dc.date.accessioned2020-06-21T14:04:12Z
dc.date.available2020-06-21T14:04:12Z
dc.date.issued2013
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[McKain] Nest, The Rowett Institute, Aberdeen, Scotland, United Kingdom; [Genç] Buǧra, Department of Animal Nutrition and Nutritional Diseases, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Snelling] Timothy John, The Rowett Institute, Aberdeen, Scotland, United Kingdom; [Wallace] Robert John, The Rowett Institute, Aberdeen, Scotland, United Kingdomen_US
dc.description.abstractBacteria and archaea in frozen (- 20. °C) ruminal digesta were analysed by qPCR and cloning/sequencing of 16S rRNA genes. Samples frozen with and without glycerol as cryoprotectant indicated a major loss of Bacteroidetes in unprotected samples, resulting in higher proportions of Firmicutes. Archaeal numbers and diversity were unaffected. © 2013 Elsevier B.V.en_US
dc.identifier.doi10.1016/j.mimet.2013.10.009
dc.identifier.endpage383en_US
dc.identifier.issn0167-7012
dc.identifier.issn1872-8359
dc.identifier.issue3en_US
dc.identifier.pmid24161897
dc.identifier.scopus2-s2.0-84887364131
dc.identifier.scopusqualityQ3
dc.identifier.startpage381en_US
dc.identifier.urihttps://doi.org/10.1016/j.mimet.2013.10.009
dc.identifier.volume95en_US
dc.identifier.wosWOS:000329003500014
dc.identifier.wosqualityQ3
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Microbiological Methodsen_US
dc.relation.journalJournal of Microbiological Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryoprotectionen_US
dc.subjectGlycerolen_US
dc.subjectRumenen_US
dc.subjectStorageen_US
dc.titleDifferential Recovery of Bacterial and Archaeal 16S rRNA Genes from Ruminal Digesta in Response to Glycerol as Cryoprotectanten_US
dc.typeArticleen_US
dspace.entity.typePublication

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