Identification of Self-Incompatibility (S) Alleles of Some Local Sweet Cherry Genotypes from Turkey

Abstract

Self-incompatibility and incompatibility groups are common in fruit species belonging to the Rosaceae family and especially in sweet cherries. There are few self-compatible sweet cherry cultivars in the world. Gametophytic self-incompatibility in sweet cherries is determined by the S-alleles. In recent years, many scientific studies have been carried out on this subject in the world, and the S-alleles associated with self-incompatibility in standard sweet cherry cultivars have been identified. This study was conducted in Ondokuz Mayis University Faculty of Agriculture, Department of Horticulture, to determine the S-alleles of 44 sweet cherry genotypes from Amasya and Giresun provinces. For the determination of S-alleles of these genotypes, standard cultivars such as Starks Gold, 0900 Ziraat and Metron Late, whose S-alleles are known, were used for control purposes. In the research, after DNA isolation of leaf samples belonging to genotypes, two forward and two reverse primers specific to S-alleles were combined with each other and PCR was performed. After PCR, the DNA bands, which were obtained as a result of electrophoresis, were interpreted. While two alleles were observed in 19 genotypes and one allele in 16 genotypes, it was observed that amplification did not occur in nine genotypes. Moreover, incompatibility groups of 13 of all genotypes were determined. While five genotypes were determined in the same incompatibility group with Metron Late, G-2 genotype in the same incompatibility group with 0900 Ziraat, five genotypes in incompatibility group III (S3S4), S-1 genotype in group XXVII, and T-3 genotype was determined in incompatibility group II.