Publication:
Analysis of Borrelia burgdorferi Proteome and Protein–Protein Interactions

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dc.authorscopusid35084558100
dc.authorscopusid15758839100
dc.authorscopusid9734419900
dc.authorscopusid24401885500
dc.authorscopusid57202809972
dc.authorscopusid55868017300
dc.authorscopusid6504499889
dc.contributor.authorYang, X.
dc.contributor.authorThakur, M.
dc.contributor.authorKoči, J.
dc.contributor.authorSmith, A.A.
dc.contributor.authorSingh, P.
dc.contributor.authorZhuang, X.
dc.contributor.authorPromnares, K.
dc.date.accessioned2020-06-21T13:13:19Z
dc.date.available2020-06-21T13:13:19Z
dc.date.issued2018
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Yang] Xiuli, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Thakur] Meghna, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Koči] Juraj, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Smith] Alexis A., Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Singh] Preeti, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Zhuang] Xuran, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Promnares] Kamoltip, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States; [Wang] Yan, Proteomics Core Facility, College of Computer, Mathematical, & Natural Sciences, College Park, MD, United States; [Büyüktanir Yaş] Özlem, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Pal] Utpal, Department of Veterinary Medicine, University of Maryland, College Park, College Park, MD, United States, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, United Statesen_US
dc.description.abstractThe proteome of Borrelia burgdorferi undergoes dynamic alterations as the microbe cycles through and persists in diverse host or vector environments. Therefore, studies of B. burgdorferi proteome and protein–protein interactions, which play central roles in biological processes in diverse organisms, are critical in understanding biology and infectivity of spirochetes. Here, we describe the proteomic analysis of B. burgdorferi by two-dimensional (2-D) gel electrophoresis followed by protein identification via liquid chromatography-mass spectrometry and database searching. We also describe assays for studying the interaction between borrelial proteins: a novel high-throughput luciferase assay, yeast two-hybrid assay, and a far-Western assay that are routinely used in our laboratories. © 2018, Springer Science+Business Media LLC.en_US
dc.identifier.doi10.1007/978-1-4939-7383-5_19
dc.identifier.endpage277en_US
dc.identifier.isbn9781597452946
dc.identifier.isbn9781617792304
dc.identifier.isbn9781617797668
dc.identifier.isbn1597455741
dc.identifier.isbn9781603272476
dc.identifier.isbn9781597453035
dc.identifier.isbn9781493912230
dc.identifier.isbn9781588298645
dc.identifier.isbn9781617793394
dc.identifier.isbn9781617799648
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid29032550
dc.identifier.scopus2-s2.0-85032028180
dc.identifier.scopusqualityQ4
dc.identifier.startpage259en_US
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-7383-5_19
dc.identifier.volume1690en_US
dc.identifier.wosWOS:000433438100021
dc.language.isoenen_US
dc.publisherHumana Press Inc. humana@humanapr.comen_US
dc.relation.ispartofMethods in Molecular Biologyen_US
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.journalBorrelia Burgdorferi: Methods and Protocolsen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBorrelia burgdorferien_US
dc.subjectFar-Westernen_US
dc.subjectLuciferase Assayen_US
dc.subjectMass Spectrometryen_US
dc.subjectProtein–Protein Interactionen_US
dc.subjectProteomics Analysisen_US
dc.subjectTwo-Dimensional (2-D) Gel Electrophoresisen_US
dc.subjectYeast Two-Hybriden_US
dc.titleAnalysis of Borrelia burgdorferi Proteome and Protein–Protein Interactionsen_US
dc.typeBook Parten_US
dspace.entity.typePublication

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