A Study on Clonal Propagation by Cutting of Two Native Boxwood Species

Abstract

The study investigated the effects of different concentrations of Indole-3-butyric acid (IBA) on the rooting of semi-wood cuttings from two endangered boxwood species, B. sempervirens and B. balearica, which are naturally found in Turkey. Given the increasing threats to these species due to habitat loss and environmental changes, effective propagation methods are crucial for their conservation. Results indicated that the application of 4000 ppm IBA significantly enhanced root length by 10.9% compared to the control group. In contrast, 2000 ppm was most effective for root width (11.3%) and callus formation (19.1%). The highest concentrations, 8000 ppm, resulted in a notable increase in root number (53.7%) and root quality (25%). This concentration also led to improvements in shoot length (3.5 cm) and number of leaf per shoot (9.3). Notably, 8000 ppm yielded a 31.6% increase in overall rooting rate, with B. balearica achieving a maximum rooting rate of 93.3% in type 4 cuttings, while B. sempervirens reached 80% in type 1 cuttings. The findings underscore that rooting characteristics vary significantly between species and types, with B. balearica demonstrating superior rooting performance compared to B. sempervirens. The positive effects of IBA on rooting highlight its potential utility in the propagation of these endangered boxwoods, contributing to landscape restoration and forest rehabilitation efforts. In conclusion, this study provides valuable insights into the optimal use of IBA concentrations for enhancing rooting success in endangered boxwood species, emphasizing the importance of phytohormone applications in plant propagation strategies aimed at conservation efforts.