Therapeutic Role of Curcumin in Oxidative DNA Damage Caused by Formaldehyde

Abstract

Purpose: Formaldehyde is a common environmental contaminant that causes oxidative DNA damage in cells by increasing the production of reactive oxygen species. The aim of this study was to investigate the amount of 8-hydroxy-deoxyguanosine (8-OhdG), tumor protein 53(TP53), beta-amyloid[Aß(1-42), Aß (1-40)], total antioxidant capacity (TAC) and malondialdehyde (MDA) and the therapeutic role of curcumin in rat cells with oxidative DNA damage caused by formaldehyde. Method: The control group was given physiological saline for 15 days (ip) and the second group was given 37% formaldehyde (ip) at a dose of 9 mg/kg group every other day. The third group was given 9 mg/kg formaldehyde (ip) every other day and treated therapeutically with 100 mg/kg curcumin every day by gavage. At the end of the trial period, urine, blood, and brain tissue was collected from the rats. Results: The levels of MDA in sera were increased and the TAC, TP53, and Aß (1-40) levels were reduced in the formaldehyde-treated group with respect to the control group (p<0.005). After treatment with curcumin, the levels of sera MDA were significantly reduced, the TAC, TP53, and Aß (1-40) levels were significantly increased (P<0.05). The levels of whole brain Aß (1-42) and 8-OhdG were increased in the formaldehyde-treated group and reduced after treatment with curcumin (P<0.05). Urinary 8-OhdG excretion increased in the formaldehyde-treated group (P<0.05) and decreased after treatment with curcumin (P>0.05). Conclusions: In conclusion, the oxidative stress caused by formaldehyde exposure was reduced with the application of curcumin. © 2015 Wiley Periodicals, Inc.