Reactive Blue Prevented Caffeine-Induced Neurotoxicity by an Independent Mechanism from Intracellular Calcium Currents in Cell Culture from Auditory Cortex of Rats

Abstract

Neurotoxicity induced by caffeine ill auditory-neuron cultures was studied ill rat pups. For possible protective effect. reactive blue (RB) alone and ill combination with dantrolene were tested in subsequent doses. RB was found to have a U-shape neuroprotective effect in caffeine neurotoxicity,. Dantrolene was also tested ill combined application in caffeine neurotoxicity. Despite the existing neuroprotection, no additional protection was obtained with various doses of dantrolene. In conclusion, RB maid exert neuroprotective effect by increasing intracellular ATP levels ill caffeine toxicity. High ATP levels may; postpone the toxic cascade. Dantrolene as an endoplasmic reticulum calcium release blocker had no additional protective effect. suggesting that the increased intracellular calcium levels may be involved ill later states of the toxic cascade, occurring after the compensatory phase of the cell death.