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Evaluation of Sperm Counting Accuracy on Computer-Assisted Sperm Analysis With Goldcyto® Slides and Glass Slides

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dc.authorscopusid55812953400
dc.authorwosidAkal, Eser/Abd-6435-2020
dc.contributor.authorAkal, Eser
dc.date.accessioned2025-12-11T00:36:19Z
dc.date.issued2023
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Akal, Eser] Ondokuz Mayis Univ, Fac Vet Med, Dept Reprod & Artificial Inseminat, Samsun, Turkiyeen_US
dc.description.abstractWorldwide, various counting chambers and computer-assisted sperm analysis (CASA) devices are in use. The semen's concentration can vary depending on the depth of the counting chamber and how it is loaded. The study's objectives were to analyze the effects of various counting chambers on semen concentration results using a GoldCyto (R) slide and a glass slide in the CASA system and to ascertain the precision of concentration measurements made using glass slides on CASA. The study's control group was composed of samples with known concentrations (72-80 million sperm/mL) as determined by a spectrophotometer. A total of 21 frozen straws from the same bull of the same date were thawed at 37degree celsius for 30 s and loaded into two different sperm-counting chambers (GoldCyto (R) slide and glass slide). The sample semen placed in the sperm counting chambers was 5 mu L and the same value was entered in the CASA software as 5 mu L. Measurements were done and evaluated in 5 different areas. According to the data we obtained, using the glass slide were statistically lower than the spectrophotometer (p < 0.001). GoldCyto (R) slide results were consistent with spectrophotometer results. Consequently, measurements with GoldCyto (R) slides in the CASA had consistent results, while measurements with glass slides were inconsistent. It was concluded that GoldCyto (R) slides are more suitable than glass slides in the concentration examinations of semen. Therefore, more study is needed to optimize the use of glass slides.en_US
dc.description.sponsorshipThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. During this study, no financial or spiritual support was received neither from any pharmaceutical company that has a direct connection wien_US
dc.description.sponsorshipThe author(s) declare financial support was received for the research, authorship, and/or publication of this article. During this study, no financial or spiritual support was received neither from any pharmaceutical company that has a direct connection with the research subject nor from a company that provides or produces medical instruments and materials which may negatively affect the evaluation process of this study.en_US
dc.description.woscitationindexScience Citation Index Expanded
dc.identifier.doi10.3389/fvets.2023.1283128
dc.identifier.issn2297-1769
dc.identifier.pmid37876632
dc.identifier.scopus2-s2.0-85174827770
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3389/fvets.2023.1283128
dc.identifier.urihttps://hdl.handle.net/20.500.12712/37787
dc.identifier.volume10en_US
dc.identifier.wosWOS:001089248100001
dc.identifier.wosqualityQ1
dc.institutionauthorAkal, Eser
dc.language.isoenen_US
dc.publisherFrontiers Media Saen_US
dc.relation.ispartofFrontiers in Veterinary Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCASAen_US
dc.subjectGlass Slideen_US
dc.subjectGoldcyto (R) Slideen_US
dc.subjectSperm Concentrationen_US
dc.subjectSpermen_US
dc.titleEvaluation of Sperm Counting Accuracy on Computer-Assisted Sperm Analysis With Goldcyto® Slides and Glass Slidesen_US
dc.typeArticleen_US
dspace.entity.typePublication

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