Streptomyces hoynatensis sp. nov., Isolated from Deep Marine Sediment

Abstract

A novel actinobacterium, strain S1412T, was isolated from a deep sediment sample, collected from the southern Black Sea coast of Turkey, and was examined using a polyphasic approach. The organism had chemical and morphological features typical of the genus Streptomyces. The cell wall of the novel strain contained LL-diaminopimelic acid. Whole-cell hydrolysates contained galactose, glucose and traces of xylose. The polar lipid profile of S1412T consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine and phosphatidylinositol, and minor amounts of phosphatidylglycerol. Strain S1412T exhibited an unusual quinone system, with the predominant compounds MK-10(H<inf>8</inf>), MK-9(H<inf>8</inf>) and MK-10(H<inf>6</inf>) and small amounts of MK-9(H<inf>6</inf>) and MK-10(H<inf>4</inf>). Major fatty acids were iso-C<inf>16: 0</inf>, iso-C<inf>16: 1</inf> H and anteiso-C<inf>17: 0</inf>. The 16S rRNA gene sequence similarities for strain S1412T with respect to the most closely related type strains of species of the genus Streptomyces were less than 97.0 %. Phenotypic data clearly distinguished the isolate from its closest relatives, Streptomyces specialis GW 41-1564T, Streptomyces mayteni YIM 60475T, Streptomyces hainanensis YIM 47672T, Streptomyces avicenniaeMCCC1A01535T and Streptomyces sedi YIM 65188T. Based on chemotaxonomic, phenotypic and genotypic characteristics, strain S1412T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces hoynatensis sp. nov. is proposed. The type strain is S1412T (= KCTC 29097T = DSM 42069T). © 2014 IUMS.