Investigation of the Presence of Lr24 Gene Providing Tolerance to Brown Rust Disease Using SCAR Marker in the Spring Bread Wheat Gene Pool

Abstract

Leaf rust is an important fungal disease in wheat and causes significant yield losses worldwide. Previous studies have reported that Lr9, Lr19, Lr24, Lr41, and Lr47 genes provide resistance to leaf rust in wheat. This study was conducted in 2022 to determine whether Alt & imath;ndane, Kirve, Pandas, Kate-A1 and G & ouml;nen-98, spring bread wheat varieties registered in T & uuml;rkiye, have the Lr24 gene that provides tolerance to brown rust disease. In the study, Sunco and Torres spring bread wheat varieties, which have the brown rust disease tolerance gene Lr24, were used. In order to detect the presence of the Lr24 gene in the wheat varieties evaluated, a SCAR (Sequence-Characterized Amplified Region) marker was used in 3 replications. As a result of the research, it was determined that none of the 5 spring wheat varieties registered in T & uuml;rkiye had the brown rust disease tolerance gene Lr24, while Torres and Sunco spring wheat varieties examined had the tolerance gene Lr24. Finally, it was concluded that one or both Torres and Sunco wheat varieties with the wheat brown rust tolerance gene Lr24 could be used as a wheat brown rust tolerance gene donor parent in a hybridization breeding program to develop new wheat varieties tolerant to brown rust disease.