Investigation of Diagnostic Use of Serum Anti-Mullerian Hormone Concentration in Dioestrus and Anoestrus Bitches Before and After Ovariohysterectomy and the Relationship with Ovarian Follicle Numbers

Abstract

The aims of the study were to (1) compare the serum concentration of anti-Mullerian hormone (AMH) with the number of follicles in ovaries and (2) determine the serum AMH concentration before and after ovariohysterectomy in dioestrus and anoestrus bitches. Sixteen bitches were divided into two groups: Group I (n=8) consisted of dioestrus and group II (n=8) anoestrus bitches. The blood samples for AMH assesment were taken before ovariohysterectomy (day 0) and on day 1, 5 and 10. Both in group I and II, serum AMH concentrations on day 1 and 5 were significantly different compared to day 0 (p<0.05). However, the concentrations at day 10 were under the minimum detectable concentration (1.0 ng/mL) and this finding revealed that ovaries are the only source of AMH synthesis. Follicle counts were not statistically different between the groups (p>0.05). Significantly positive correlation in serum AMH with secondary follicle numbers (r=.942, p<0.01), as well as negative correlation with antral follicle numbers (r=-.765, p<0.05) were determined in the group I. In the group II, positive correlations between serum AMH concentration and secondary follicle numbers (r=.960, p<0.01) and early antral follicles (r=.726, p<0.05) were noted. Assesment of AMH concentration seems to not only provide the diagnosis of the presence of ovaries but also correlate with the number of secondary follicles in young dioestrus and anoestrus bitches.