Evaluation of Age-Related Spermatozoa Morphological Section Lengths in Gloster Canary Spermatozoa using Computer Aided Sperm Analysis (AACS) System

Abstract

The canary is a small passerine bird of the finch family. Canary spermatozoa are also morphologically similar many other songbird species. In this study, the morphological characteristics of Gloster canary spermatozoa of different age ranges were examined, and the length values of the parts forming the spermatozoa were compared. The study ten active Gloster canaries between the ages of three (Trial-1) and four years (Trial-2). Semen samples from 3-year canaries in the study were evaluated, and morphological segment lengths were recorded. In the study, to individual differences and make homogeneous measurements, semen samples were retaken from the same canaries one year later during the breeding period and evaluated. However, since the statistical average result was obtained the study, the canaries were compared as a whole, not individually. Semen was collected from each bird and fixed 5% formaldehyde solution. Collected semen samples were evaluated in terms of head length, total flagellum acrosome length, and nucleus size at 20x magnification in the computer-assisted CASA system for morphological examination. As a result, it was observed that the head and flagellum length in domestic canary spermatozoa showed an inverse relationship with age.