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Conventional and Molecular Biotyping of Brucella Strains Isolated from Cattle, Sheep and Human

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dc.authorscopusid16646201600
dc.authorscopusid16644970500
dc.authorscopusid6603130380
dc.authorscopusid33568235700
dc.authorscopusid6504180301
dc.authorscopusid15137077000
dc.authorscopusid32867692200
dc.contributor.authorİça, T.
dc.contributor.authorAydin, F.
dc.contributor.authorGümüşsoy, K.S.
dc.contributor.authorPerçin, D.
dc.contributor.authorSümerkan, A.B.
dc.contributor.authorOcak, F.
dc.contributor.authorAbay, S.
dc.date.accessioned2020-06-21T14:28:31Z
dc.date.available2020-06-21T14:28:31Z
dc.date.issued2012
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[İça] Tuba, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Aydin] Fuat, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Gümüşsoy] Kadir Semih, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Perçin] Duygu Eşel, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Sümerkan] Ahmet Bülent, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Ocak] Fulya, Department of Biology, Manisa Celâl Bayar Üniversitesi, Manisa, Manisa, Turkey; [Abay] Seçil, Department of Microbiology, Erciyes Üniversitesi, Kayseri, Kayseri, Turkey; [Doǧan] Halef Okan, Laboratory of Biochemistry, Ankara Numune Education and Research Hospital, Ankara, Turkey; [Findik] Arzu, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Çiftçi] Alper, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkeyen_US
dc.description.abstractIn this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods. For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined. A total of 29 Brucella spp. 17 (27.9%) and 12 (18.7%) of which were isolated from cattle and sheep specimens, respectively) were isolated from animal sources. Both animal and human isolates were typed by conventional and Enhanced AMOS-ERY PCR methods. All Brucella spp. strains isolated from cattle were found to belong to B. abortus biovar 3 and biovar 3b using conventional and molecular typing methods, respectively. All sheep originated Brucella spp. strains and human originated Brucella spp. strains were found to belong to B.melitensis biovar 3 using both conventional and molecular methods. As a result, predominant biovars causing brucellosis in human, cattle and sheep in Kayseri, Turkey were detected. These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey.en_US
dc.identifier.doi10.1501/Vetfak_0000002536
dc.identifier.endpage264en_US
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84904267590
dc.identifier.scopusqualityQ3
dc.identifier.startpage259en_US
dc.identifier.urihttps://doi.org/10.1501/Vetfak_0000002536
dc.identifier.volume59en_US
dc.identifier.wosWOS:000309621500005
dc.identifier.wosqualityQ3
dc.language.isoenen_US
dc.publisherChartered Inst. of Building Services Engineers hq@cibse.orgen_US
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.journalAnkara Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiotypingen_US
dc.subjectBrucellaen_US
dc.subjectEnhanced AMOS-Ery PCRen_US
dc.titleConventional and Molecular Biotyping of Brucella Strains Isolated from Cattle, Sheep and Humanen_US
dc.title.alternativeSığır, Koyun ve İnsanlardan İzole Edilen Brucella Suşlarının Konvansiyonel ve Moleküler Biyotiplendirmesien_US
dc.typeArticleen_US
dspace.entity.typePublication

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