Streptomyces klenkii sp. nov., Isolated From Deep Marine Sediment

Abstract

A novel actinomycete strain, designated S2704T, was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey and characterized using a polyphasic approach. Comparison of 16S rRNA gene sequences showed that strain S2704T is a member of the genus Streptomyces, exhibiting highest similarity with Streptomyces specialis GW 41-1564T (97.1 %). Strain S2704T could be distinguished from all other Streptomyces species with validly published names by sequence similarity values less than 97.0 %. The isolate was found to contain LL-diaminopimelic acid as the diagnostic diamino acid. Whole cell hydrolysates were found to contain glucose, mannose and ribose. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, glycophospholipid, a phospholipid and two unknown glycolipids. The predominant menaquinones were identified as MK-9(H<inf>8</inf>) and MK-10(H<inf>6</inf>). The major fatty acids were found to be anteiso-C<inf>17:0</inf>, iso-C<inf>16:0</inf> and anteiso-C<inf>15:0</inf>. The G+C content of the DNA was determined to be 72.2 mol%. On the basis of its phenotypic and molecular properties, strain S2704T is considered to be a novel species of the genus Streptomyces, for which the name Streptomyces klenkii sp. nov. is proposed. The type strain is S2704T (=DSM 42104T = KCTC 29202T). © 2014, Springer International Publishing Switzerland.