Biological Control of Heterodera Cruciferae (Tylenchida: Heteroderidae) Franklin 1945 with Fluorescent Pseudomonas spp

Abstract

This study was conducted to access the ability of fluorescent Pseudomonas spp. to suppress cyst nematode Heterodera cruciferae, which was caused by reproduction. The yield loss of cruciferous plants in infested soil with H. cruciferae was examined in in vitro and in vivo experiments. For this purpose, fluorescent Pseudomonas spp. was isolated from infested soil with cyst nematode H. cruciferae and a selected strain of fluorescent Pseudomonas [Fluorescent Pseudomonas strain no. 6 (FPs6)] used on cyst, females and eggs of H. cruciferae to study the biological control. In the in vitro study, eggs of H. cruciferae were infected by FPs6 on King's B Agar and consequently the growth and hatching of eggs were inhibited. Furthermore, J1 and J2 being grown in diseased eggs as they were infected by FPs6. In the in vivo study, plant lengths, leaf areas, wet weights, dry weights and root lengths of seedlings were investigated. There were no considerable differences among FPs6, FsP6 + Cyst and Control groups on plant length and root length (P > 0.05). But there were considerable differences between Cyst and Control groups on plant length and root length (P > 0.05).