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Generation of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterization

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dc.authorscopusid6504499889
dc.authorscopusid56571527200
dc.authorscopusid32867692200
dc.authorscopusid10040320700
dc.authorscopusid22946038300
dc.contributor.authorBüyüktanir Yaş, O.
dc.contributor.authorYaǧci, T.
dc.contributor.authorFindik, A.
dc.contributor.authorYildirim, T.
dc.contributor.authorYurdusev, N.
dc.date.accessioned2025-12-10T22:13:22Z
dc.date.issued2011
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Büyüktanir Yaş] Özlem, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Yaǧci] Tamer, Department of Molecular Biology and Genetics, Bilkent Üniversitesi, Ankara, Ankara, Turkey; [Findik] Arzu, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Yildirim] Tuba, Department of Biology, Amasya Üniversitesi, Amasya, Turkey; [Yurdusev] Nevzat, Department of Microbiology, Ondokuz Mayis Üniversitesi, Samsun, Turkeyen_US
dc.description.abstractBALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody. The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myeloma cells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to be reactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDa molecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessary to obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.en_US
dc.identifier.endpage670en_US
dc.identifier.issn1300-6045
dc.identifier.issn1309-2251
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-79959584919
dc.identifier.scopusqualityQ3
dc.identifier.startpage667en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12712/35009
dc.identifier.volume17en_US
dc.identifier.wosqualityQ3
dc.language.isotren_US
dc.publisherVeteriner Fakultesi Dergisi vetdergi@kafkas.edu.tren_US
dc.relation.ispartofKafkas Üniversitesi Veteriner Fakültesi Derneğien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCell Fusionen_US
dc.subjectHybridomaen_US
dc.subjectSalmonella Enteritidisen_US
dc.subjectSpecific Antibodyen_US
dc.titleGeneration of Mouse Hybridomas Secreting Anti-Salmonella Enteritidis Antibodies and Their Preliminary Characterizationen_US
dc.title.alternativeAnti-salmonella Enteritidis Antikorları Salgılayan Fare Hibridomalarının Geliştirilmesi ve Ön Karakterizasyonlarıen_US
dc.typeArticleen_US
dspace.entity.typePublication

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