Publication: Evaluation of Fluorescence in Situ Hybridization (FISH) and Polymerase Chain Reaction (PCR) for Identification of Enterococcus spp. in Fish and Water
| dc.contributor.author | Ozer, Selmin | |
| dc.contributor.author | Borekci, Gulay | |
| dc.contributor.author | Bulduklu, Pinar | |
| dc.contributor.author | Ciftci, Alper | |
| dc.contributor.author | Kanik, Arzu | |
| dc.contributor.author | Onuk, Ertan Emek | |
| dc.date.accessioned | 2020-06-21T14:46:22Z | |
| dc.date.available | 2020-06-21T14:46:22Z | |
| dc.date.issued | 2011 | |
| dc.department | OMÜ | en_US |
| dc.department-temp | [Ozer, Selmin -- Bulduklu, Pinar] Mersin Univ, Fac Fisheries, Dept Aquaculture, TR-33169 Mersin, Turkey -- [Borekci, Gulay] Mersin Univ, Hlth Sch, TR-33169 Mersin, Turkey -- [Ciftci, Alper] Ondokuz Mayis Univ, Fac Vet, Dept Microbiol, TR-55139 Kurupelit, Samsun, Turkey -- [Kanik, Arzu] Mersin Univ, Fac Med, Dept Biostat, TR-33169 Mersin, Turkey -- [Onuk, Ertan Emek] Ondokuz Mayis Univ, Fac Vet, Dept Fisheries & Fish Dis, TR-55139 Kurupelit, Samsun, Turkey -- | en_US |
| dc.description.abstract | Three methods for identifying Enterococcus spp. and E. faecalis were compared. Using conventional culture methods, 99 isolates from rainbow trout (Oncorhynchus mykiss Walbaum) and water samples were identified as Enterococcus spp. and 86.7% of these were identified as E. faecalis. Using the oligonucleotide probes Eub338, NonEub, Enc145, Efs129, and Str, fluorescence in situ hybridization (FISH) identified 73.7% of the 99 Enterococcus spp. as Enterococcus spp. and 58.6% as E. faecalis. Using polymerase chain reaction (PCR) methods, 88.9% of the 99 samples were identified as Enterococcus spp., 36.4% as E. faecalis, and none as E. faecium. Thus, the consistency between conventional, FISH, and PCR methods was inadequate. | en_US |
| dc.description.sponsorship | Unit of Scientific Research Projects of the University of MersinMersin University [BAP-SUF-YB (SO) 2007-1] | en_US |
| dc.description.sponsorship | This study was supported by the Unit of Scientific Research Projects of the University of Mersin; with project number BAP-SUF-YB (SO) 2007-1. We would like to thank Prof. Dr. Gurol Emekdas, Prof. Dr. Gonul Aslan, and Assoc. Prof. Dr. Serap Yalin for their technical support. | en_US |
| dc.identifier.issn | 0792-156X | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12712/17522 | |
| dc.identifier.volume | 63 | en_US |
| dc.identifier.wos | WOS:000296497300001 | |
| dc.language.iso | en | en_US |
| dc.publisher | Israeli Journal of Aquaculture-Bamidgeh | en_US |
| dc.relation.journal | Israeli Journal of Aquaculture-Bamidgeh | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | FISH | en_US |
| dc.subject | PCR | en_US |
| dc.subject | Enterococcus spp. | en_US |
| dc.title | Evaluation of Fluorescence in Situ Hybridization (FISH) and Polymerase Chain Reaction (PCR) for Identification of Enterococcus spp. in Fish and Water | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication |