Publication: İtalyan Çimi ve Çayır Üçgülü Karışımına İlave Edilen Laktik Asit Bakterileri ve Enzim İçeren İnokulantın Silaj Kalitesi ve Sindirilebilirliği Üzerine Etkisi
Abstract
Bu çalışmanın amacı, İtalyan çimi-çayır üçgülü karışımından oluşan silaj materyaline laktik asit bakterileri+enzim (LABE) katkı maddesinin inokülasyonunun silajın besin madde bileşimi, kimyasal özellikleri ve in vitro sindirilebilirlik değerleri üzerine etkisini belirlemektir. Araştırmada, kontrol grubunun silajlarına LABE inokulantı eklenmezken, LABE1 ve LABE2 gruplarına sırasıyla 150 000 ve 300 000 kob/g silaj materyali olacak şekilde LABE eklenmiş ve 60 gün inkübe edilmiştir. Her iki biçimde de LABE1 ve LABE2 silajlarının pH değeri kontrol grubu silajlarına göre daha düşük (P<0,05) bulunmuştur. Birinci biçim İtalyan çimi-çayır üçgülü silajlarında en yüksek laktik asit değeri LABE2 grubu silajlarında tespit edilirken, ikinci biçim İtalyan çimi-çayır üçgülü silajlarının laktik asit değerleri LABE katkılı gruplarda en yüksek (P<0,05) bulunmuştur. Birinci biçim İtalyan çimi-çayır üçgülü silajlarının amonyak azotu konsantrasyonları yönünden gruplar arasında bir farklılık saptanmazken, ikinci biçim İtalyan çimi-çayır üçgülü silajlarında amonyak azotu konsantrasyonu LABE2 grubunda kontrol ve LABE1 grubuna göre daha yüksek (P<0,05) bulunmuştur. Her iki biçimde de LABE ilavesi silajların in vitro sindirilebilirlik değerlerinde artışlar (P<0,05) ile sonuçlanmıştır. Her iki biçimde LABE gruplarında aerob mezofil genel canlı ve enterekok populasyonu azalmış, (P<0,05), laktik asit bakterilerinde değişiklik saptanmamış ve maya-küf populasyonu <2,3 log10 kob/g tespit edilmiştir. Sonuç olarak, İtalyan çimi-çayır üçgülü hasılına 300 000 kob/g'a kadar LABE ilavesinin her iki düzeyinin de silaj kalitesini ve sindirilebilirlik değerlerini artırdığı sonucuna varılmıştır.
The aim of this study was to determine the effect of inoculation of lactic acid bacteria+enzyme (LABE) additive to the silage material consisting of İtalian ryegrass-red clover mixture on nutrient composition, chemical properties and in vitro digestibility values of silage. In the study, while no LABE inoculant was added to the silages of the control group, LABE was added as 150 000 and 300 000 cfu/g of silage material for LABE1 and LABE2 groups, respectively, and incubated for 60 days. The pH values of LABE1 and LABE2 silages were lower (P<0.05) than the control silages for the first and second harvests. While the highest lactic acid value in the first harvest Italian ryegrass-red clover silages was detected in the LABE2 group silages, the lactic acid values of the second harvest Italian ryegrass-red clover silages were the highest (P<0.05) in the groups with LABE. While there was no difference between groups in terms of ammonia nitrogen concentrations of the first harvest Italian ryegrass-red clover silages, the ammonia nitrogen concentration in the second harvest Italian ryegrass-red clover silages was higher (P<0.05) in the LABE2 group than the others. In both harvests, LABE inoculation resulted in increases (P<0.05) in the in vitro digestibility values of silages. In both harvests, aerob mesophyll general viability and enterococci population decreased (P<0.05), lactic acid bacteria did not change and yeast-mold population was <2.3 log10 cfu/g in LABE groups. As a result, both levels of the addition of LABE up to 300 000 cfu/g to the Italian ryegrass-red clover mixture increased silage quality and digestibility values.
The aim of this study was to determine the effect of inoculation of lactic acid bacteria+enzyme (LABE) additive to the silage material consisting of İtalian ryegrass-red clover mixture on nutrient composition, chemical properties and in vitro digestibility values of silage. In the study, while no LABE inoculant was added to the silages of the control group, LABE was added as 150 000 and 300 000 cfu/g of silage material for LABE1 and LABE2 groups, respectively, and incubated for 60 days. The pH values of LABE1 and LABE2 silages were lower (P<0.05) than the control silages for the first and second harvests. While the highest lactic acid value in the first harvest Italian ryegrass-red clover silages was detected in the LABE2 group silages, the lactic acid values of the second harvest Italian ryegrass-red clover silages were the highest (P<0.05) in the groups with LABE. While there was no difference between groups in terms of ammonia nitrogen concentrations of the first harvest Italian ryegrass-red clover silages, the ammonia nitrogen concentration in the second harvest Italian ryegrass-red clover silages was higher (P<0.05) in the LABE2 group than the others. In both harvests, LABE inoculation resulted in increases (P<0.05) in the in vitro digestibility values of silages. In both harvests, aerob mesophyll general viability and enterococci population decreased (P<0.05), lactic acid bacteria did not change and yeast-mold population was <2.3 log10 cfu/g in LABE groups. As a result, both levels of the addition of LABE up to 300 000 cfu/g to the Italian ryegrass-red clover mixture increased silage quality and digestibility values.
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