Determination of Helicobacter Infections With 14C Urea Breath Test (14C UBT) and Polymerase Chain Reaction (PCR) in Dogs and Treatment

Abstract

It was aimed to determine that if14C UBT (14C Urea Breath Test) application as a “Gold Standard” in the human practice for diagnosing Helicobacter infections could also be used in an easy, practical, and a reliable method in the veterinary practice. Three groups were allocated in the study. Dogs (n=41) were detected as clinically healthy in their physical examinations were grouped as healthy (Group I). Dogs (n=32) had clinical symptoms of vomiting after feeding, anorexia, epigastric pain during abdominal palpation were grouped as diseased and these diseased dogs (Group II) that were treated grouped as treatment (n=32) (Group III). Helicobacter staining, Polymerase Chain Reaction (PCR) and14C UBT were compared for diagnosis of Helicobacteriosis before and after therapy. Based on the results of the present study14C UBT was found 2 times more reliable when compared to PCR and 65 times more reliable than Helicobacter staining technique. Sensitivity for14C UBT, PCR and Helicobacter staining technique was found as 96.55%, 93.75%, and 59.38%, respectively. Moreover specificity of the14C UBT, PCR and Helicobacter staining technique was detected as 97.73%, 97.56% and 92.68%, respectively. Consequently, it was thought that14C UBT test could be used as a reliable method in veterinary practice. © 2015, KAFKAS UNIVERSITY. All rights received.