Production Capabilities of Transglutaminase, Levansucrase and Beta Galactosidase of Actinobacteria Isolates

Abstract

Actinobacteria occupy an important position among alternative enzyme sources with their ability to growth in extreme conditions, their potential to produce large amounts of enzymes, biochemical diversity and compatibility with genetic manipulations. In this study, the selection of suitable producer microorganisms for transglutaminase, ß-galactosidase and levansucrase enzymes was aimed, and the enzyme production capabilities of 46 actinobacterial isolates were determined. The ability of these actinobacterial isolates to produce related enzymes was determined by scanning the genome sequences deposited in “GenBank using Rapid Annotation using Subsystem Technology Version 2.0”. Subsequently, the enzyme production capabilities of these isolates with relevant gene were determined by Hydroximate Method for transglutaminase (with the Paper Disc Method), the ONPG method for ß-galactosidase and the mucoid structure formation phenotype for levansucrase. By bioinformatics scanning, it was determined that all isolates contained a “transglutaminase-like enzyme” gene region and by qualitative screening, 9 isolates with different species and that can grow rapidly in medium were identified as potential isolates. A levansucrase-production gene was determined in only 2 isolates (Micromonospora sp. KC 721 and Micromonospora sp. KC213) but none of these showed activity in agar and broth medium. The presence of ß-galactosidase enzyme production gene was detected in 38 isolates. As a result of the qualitative test, 17 isolates with more intense color, different species and that can grow rapidly in medium were selected as potential ß-galactosidase-producers and determined as actinobacterial isolates with a potential to be used in industrial scale enzyme production for different biotechnological applications. © 2022 by the authors.