Comparative Cranial Geometric Morphometrics Among Wistar Albino, Sprague Dawley, and Wag/Rij Rat Strains

Abstract

Simple Summary Geometric morphometrics allows the examination of the shape variation of structures. In this study, the skull, mandible, and teeth of three different strains of rats were examined. The results showed that the strains significantly differed in shape. The most important shape difference in the skull was the variation of the cranium from an oval to an elongated structure. In this respect, Sprague Dawley rats showed a more elongated skull, while WAG/Rij rats had a more oval skull. Wistar Albino rats showed a more moderate shape variation. WAG/Rij rats showed very different shapes of the mandible and teeth compared to the other strains. But statistically, it was seen that all strains were completely separated from each other.Abstract This research utilizes geometric morphometrics to investigate shape variation in the skull, mandible, and teeth among three rat strains: Wistar Albino (WA), Sprague Dawley (SD), and WAG/Rij (WR). Through the analysis of 48 rats using 2D geometric morphometric techniques, significant differences in their skull morphology were identified. This study indicates a shift from a rectangular to an oval cranial shape across strains, with notable size and morphological variances. Particularly, the WR strain's skull shape significantly differs from the SD and WA strains, suggesting distinct ecological or genetic pathways. Compared to the skull, mandible shape differences are less pronounced, but still significant. The WR strain exhibits a distinct mandible shape, potentially reflecting ecological adaptations like dietary habits. The teeth shape of WR rats is the most distinct. SD rats consistently exhibited larger sizes in both skull and mandible measurements, while WR rats were notably smaller. Interestingly, sexual dimorphism was not statistically significant in skull and teeth sizes, aligning with findings from previous studies. However, the mandible showed clear size differences between sexes, underscoring its potential for adaptive or behavioral studies. In summary, this study provides a comprehensive analysis of morphological variations in rat strains, highlighting the intricate interplay of size, shape, and ecological factors. These findings lay a foundation for deeper explorations into the adaptive, ecological, or genetic narratives influencing rat morphology.