A Novel Biomarker Explaining the Role of Oxidative Stress in Exercise and L-Tyrosine Supplementation: Thiol/Disulphide Homeostasis

Abstract

The present study aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using thiol/disulphide homeostasis via a novel biomarker in rats. Following the completion of the exercise and l-tyrosine protocol, serum total thiol, native thiol, and disulphide concentrations were determined using a novel automated measurement method. Compared with the control group, serum dynamic disulphide levels were significantly lower in the E group (116.75 ± 10.49; p <.05) and the highest in the LT group (151.0 ± 5.84). The lowest oxidised thiol (49.75 ± 6.18; p =.087) and the highest reduced thiol (75.38 ± 3.16; p =.079) rates were determined to be in the E group. The highest oxidised thiol value was observed in the LT group. Exercise positively affects thiol/disulphide homeostasis, which is a novel indicator of oxidant-antioxidant parameters. Additionally, l-tyrosine appears to be more convenient combined with exercise. The new method used in our study proposes a promising, practical, and useful method for assessing the oxidative stress parameters. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.