Genotyping via Sequence Related Amplified Polymorphism Markers in Fusarium Culmorum

Abstract

Fusarium culmorum is predominating causal agent of head blight (HB) and root rot (RR)in cereals worldwide. Since F. culmorum has a great level of genetic diversity and theparasexual stage is assumed for this phytopathogen, characterization of isolates fromdifferent regions is significant step in food safety and controlling the HB. In this study, itwas aimed to characterize totally 37 F. culmorum isolates from Turkey via sequencerelated amplified polymorphism (SRAP) marker based genotyping. MAT-1/MAT-2 typeassay was also used in order to reveal intraspecific variation in F. culmorum. MAT-1 andMAT-2 specific primer pairs for mating assays resulted in 210 and 260 bp bands,respectively. 11 of isolates were belonged to MAT-1 type whereas 19 samples were ofMAT-2. Remaining 7 samples yielded both amplicons. Totally 9 SRAP primer setsyielded amplicons from all isolates. Genetic similarity values were ranged from 39 to94.7%. Total band number was 127 and PCR product sizes were in the range of 0.1-2.5kb. Amplicon numbers for individuals were ranged from 1 to 16. According to dataobtained from current study, SRAP based genotyping is powerful tool for supporting thedata obtained from investigations including phenotypic and agro-ecologicalcharacteristics. Findings showed that SRAP-based markers could be useful in F.culmorum characterization.