Actinomadura geliboluensis sp. nov., Isolated from Soil

Abstract

A novel actinobacterium, strain A8036T, isolated from soil, was investigated by using a polyphasic taxonomic approach. The organism formed extensively branched substrate hyphae that generated spiral chains of spores with irregular surfaces. The cell wall contained meso-diaminopimelic acid (type III) and cell-wall sugars were glucose, madurose, mannose and ribose. The predominant menaquinones were MK-9(H<inf>6</inf>) and MK-9(H<inf>4</inf>). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C<inf>16: 0</inf>, <inf>C17: 1</inf>cis9, C<inf>16: 0</inf>, C15: 0 and 10-methyl C<inf>17: 0</inf>. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of strain A8036T were Actinomadura meyerae DSM 44715T (99.23% similarity), Actinomadura bangladeshensis DSM 45347T (98.9 %) and Actinomadura chokoriensis DSM 45346T (98.3 %). However, DNA-DNA relatedness and phenotypic data demonstrated that strain A8036T could be clearly distinguished from the type strains of all closely related Actinomadura species. Strain A8036T is therefore considered to represent a novel species of the genus Actinomadura, for which the name Actinomadura geliboluensis sp. nov. is proposed. The type strain is A8036T (5DSM 45508T5KCTC 19868T). © 2012 IUMS.