Phylogeny of Plant Growth-Promoting Actinobacteria Isolated From Legume Nodules in Turkey

Abstract

Actinobacteria are a biotechnologically important group of microorganisms utilized for their high capacity to synthesize many bioactive substances as well as agriculturally important compounds. In the present study, a culture-dependant approach was employed to isolate actinobacteria from wild legume nodules and their plant growth-promoting activities for indole-3-acetic acid production, atmospheric nitrogen fixation and inorganic phosphate solubilisation was investigated. A molecular approach based on 16S rRNA gene sequence analysis was employed to identify the isolates. After pairwise sequence analysis, six isolates were identified as members of the genera Streptomyces and Micromonospora. All isolates could produce indole-3-acetic acid and utilize atmospheric nitrogen while only one isolate was able to solubilize inorganic phosphate. The isolated actinobacteria are considered to be promising candidates for biological fertilizers especially because of their ability to use atmospheric nitrogen and produce high level of indole-3-acetic acid. © 2020, Centenary University. All rights reserved.