Streptomyces karpasiensis sp. nov., Isolated from Soil

Abstract

A novel actinobacteria, designated strain K413T, was isolated from soil collected from Karpaz National Park, Magusa, Northern Cyprus, and characterized to determine its taxonomic position. The isolate was found to have chemical and morphological properties associated with members of the genus Streptomyces. Phylogenetic analyses based on almost-complete 16S rRNA gene sequences indicated that the isolate was closely related to members of the genus Streptomyces, and was shown to form a distinct phyletic line in the Streptomyces phylogenetic tree. Strain K413T was most closely related to Streptomyces marinus DSM 41968T (98.01 %). Sequence similarities with other strains of the genus Streptomyces were below 98.0 %. The cell wall of the novel strain contained LL-diaminopimelic acid. The predominant menaquinone was MK-9(H<inf>8</inf>) (45.0 %). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and phosphatidylinositol. The major fatty acids were anteiso-C<inf>15: 0</inf>, iso-C<inf>16: 0</inf> and anteiso-C<inf>17: 0</inf>. Based on 16S rRNA gene sequence analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain K413T represents a novel species of the genus Streptomyces, for which the name Streptomyces karpasiensis sp. nov. is proposed. The type strain is K413T (= KCTC 29096T = DSM 42068T). © 2014 IUMS.