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Assessment of Metal Inhibition of Antioxidant Enzyme Glutathione Reductase from Rainbow Trout Liver

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dc.authorscopusid23027537500
dc.authorscopusid23013520200
dc.contributor.authorEkinci, D.
dc.contributor.authorŞentürk, M.
dc.date.accessioned2020-06-21T14:16:27Z
dc.date.available2020-06-21T14:16:27Z
dc.date.issued2013
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Ekinci] Deniz, Department of Agricultural Biotechnology, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Şentürk] Murat, Department of Chemistry, Aǧrı İbrahim Çeçen Üniversitesi, Agri, Agri, Turkeyen_US
dc.description.abstractEffects of Co+2, Zn+2, Ca+2, Fe +2, Mn+2, Cr+3, Sn+2 and Mg +2 exposure of rainbow trout liver on the enzymatic activity of glutathione reductase (GR) were investigated. GR was purified from rainbow trout liver 1419-fold with a yield of 38.41% and a specific activity of 22.846 U/mg protein. Following enzyme isolation, inhibitory effects of Co+2, Zn+2, Ca+2, Fe+2, Mn+2, Cr +3 and Sn+2 were analysed. IC50 values of the metal ions ranged from 42.2 to 657 μM, and the Ki constants ranged from 24.2 to 567μ. Co+2, Zn+2, Fe+2 and Cr+3 exhibited competitive inhibition whereas others inhibited the enzyme in non-competitive manner. Cobalt was the most powerful inhibitor among others having the lowest IC50 and Ki values. Magnesium exhibited activatory effect on the enzyme. © 2013 Informa UK, Ltd.en_US
dc.identifier.doi10.3109/14756366.2011.615745
dc.identifier.endpage15en_US
dc.identifier.issn1475-6366
dc.identifier.issn1475-6374
dc.identifier.issue1en_US
dc.identifier.pmid21985469
dc.identifier.scopus2-s2.0-84874154215
dc.identifier.scopusqualityQ1
dc.identifier.startpage11en_US
dc.identifier.urihttps://doi.org/10.3109/14756366.2011.615745
dc.identifier.urihttps://hdl.handle.net/20.500.12712/16014
dc.identifier.volume28en_US
dc.identifier.wosWOS:000313663400002
dc.identifier.wosqualityQ1
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofJournal of Enzyme Inhibition and Medicinal Chemistryen_US
dc.relation.journalJournal of Enzyme Inhibition and Medicinal Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGlutathione Reductaseen_US
dc.subjectInhibitoren_US
dc.subjectMetal Ionen_US
dc.subjectRainbow Trouten_US
dc.titleAssessment of Metal Inhibition of Antioxidant Enzyme Glutathione Reductase from Rainbow Trout Liveren_US
dc.typeArticleen_US
dspace.entity.typePublication

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