Saccharopolyspora hattusasensis sp. nov., Isolated from Soil

Abstract

A Saccharopolyspora strain, designated CR3506 T , isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395 T (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798 T (98.4%) and Saccharopolyspora shandongensis 88 T (98.1%); low levels of DNA–DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (<50%). Strain CR3506 T was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (>10%) were MK-9(H <inf>4</inf> ) and MK-8(H <inf>4</inf> ). Major fatty acids were (>10%) iso-C <inf>16:0</inf> , C <inf>15:0</inf> 3OH, C <inf>18:0</inf> and iso-C <inf>15:0</inf> . Further, the morphological, physiological and biochemical characteristics of strain CR3506 T are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0–98.5%). Strain CR3506 T has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506 T represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506 T (=KCTC 29104 T = DSM 45715 T ). © 2017, Springer International Publishing AG.