Anti-Oxidant, DNA-Damage Protection and Anti-Cancer Properties of N-Butanol Extract of the Endemic Perralderia Coronopifolia

Abstract

This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anticancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H<inf>2</inf>O<inf>2</inf>-induced oxidative damage. The anti-proliferative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH? scavenging ability with IC<inf>50</inf>= 7.02 ± 0.02 µg/ mL. As it shown an oxidative plasmid DNA-damage inhibition against UVphotolysis of H<inf>2</inf>O<inf>2</inf>, an anti-cancer activity at higher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness. © 2018, Bangladesh Pharmacological Society. All rights reserved.