Investigation of Acute Phase Proteins in Cattle Infected With Mycobacterium Bovis

Abstract

Tuberculosis (TB) is a contagious and zoonotic disease that adversely affects human and animal health, caused by the formation of tubercules in a caseous character in the lungs, other tissues and organs. The causative of the disease is Mycobacterium bovis. An increase or decrease in acute phase protein (APP) levels is observed in various bacterial, viral and parasitic diseases in cattle. Therefore, in this study, it was aimed to determine the changes in the lev-els of APPs in cattle infected with M. bovis. In the study, 26 tuberculosis suspected and 10 healthy cattle blood serums collected from various enterprises in Samsun were used. Whole blood samples were subjected to gamma interferon ELISA test by drawing blood from the vena jugularis of cattle into tubes containing heparin. Haptoglobin (Hp), serum amyloid A (SAA), albumin and total protein (TP) levels, which are among the acute phase proteins, were determined in the blood serums taken from the groups. As a result of the analysis, it was found haptoglobin 1.166 g/L, SAA 40.353 mu g/mL, total protein 73.2 mg/dL and albumin 3.703 mg/dL in the group infected with M. Bovis, while it was found haptoglobin 0.235 g/L, SAA 20.300 mu g/mL, total protein 20.37 mg/dL and albumin 3.71 mg/dL in the healthy group. When the APP levels of the cattle infected with M. bovis and the cattle in the healthy group were compared, it was determined that haptoglobin (p < 0.01), serum amyloid A (p < 0.01) and total protein (p < 0.01) levels, which are APPs increased statistically significantly compared to the healthy group, while the albumin (p > 0.05) level decreased, but there was no statistically significant difference.