The Comparative Analysis of Phenotypic and Genotypic Properties of Aeromonas sobria Strains Isolated from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1972)

Abstract

Phenotypic and molecular characterization of Aeromonas sobria (A. sobria) isolates by antibiotyping, sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of whole cell proteins, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was aimed in this study. For this aim, thirty-six A. sobria isolates were analysed. Isolates were divided into 12 diferent antibiotypes and 4 proteotypes according to their antibiotic susceptibilities and SDS-PAGE patterns, respectively. Thirteen RAPD types were observed among all isolates. In conclusion, the use of double or triple combination of typing methods in this study was found to be more useful for discriminating the strains. The results obtained from this study may give information about phenotypic and genotypic variability of the A. sobria strains isolated from diferent regions of Turkey and can be helpful to control disease in fish through guiding the antibiotic therapy and giving information that will be useful to development vaccine. © 2015, Veteriner Fakultesi Dergisi. All rights reserved.