HLA-B27 and CYP2D6*4 Polymorphism Prevalence Analysis in Turkey

Abstract

Background: HLA-B27 positivity is held responsible for the etiopathogenesis of spondyloarthropathies, some autoinflammatory and neurological diseases, and different types of cancer. CYP2D6 from the cytochrome P450 subfamily group is involved in the metabolism of 25% of the drugs used in the treatment. Knowing that CYP2D6*4 polymorphic alleles may cause different effects on therapeutic drugs, these variant alleles are noteworthy in the lack of response to treatment, observation of adverse drug effects, as well as in the investigation of the etiopathogenesis of many diseases. In this study, we investigated the prevalence of HLA-B27 and CYP2D6*4 in the central Black Sea Region of Turkey for the analysis of ethnicity-related genetic differences in polymorphic cytochrome P450 enzymes and CYP2D6*4 alleles. Material and Methods: A total of 1063 healthy individuals were included in the study and HLA-B27 and CYP2D6*4 polymorphisms were analyzed. Genes-4U kit and Tib-Molbiol kit were used. For analysis LightCycler 480 II Real Time PCR thermal cycler was used. Results: A total of 1063 samples were studied in the HLA- B27 and CYP2D6*4 polymorphism analysis. HLA-B27 polymorphism-positivity was detected in 86 (8.9%) samples. In CYP2D6*4 polymorphism heterozygous alleles were detected in 203 (19.09%) and homozygous alleles in 26 (2.44%) samples. While 29 (2.71%) samples carried both HLA-B27 and CYP2D6*4 polymorphisms. Conclusion: In our study, we determined the frequency of polymorphism and allele frequencies at a rate similar to the data of other healthy populations.