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Effects of Dichlorvos and Characterization of Microsomal NADPH-Cytochrome P450 and NADH-Ferricyanide Reductase in Rainbow Trout (Oncorhynchus Mykiss) Liver

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dc.authorscopusid55939045800
dc.authorscopusid7004363915
dc.contributor.authorÖzen, T.
dc.contributor.authorKorkmaz, H.
dc.date.accessioned2020-06-21T15:43:17Z
dc.date.available2020-06-21T15:43:17Z
dc.date.issued2004
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Özen] Tevfik, Department of Chemistry, Ondokuz Mayis Üniversitesi, Samsun, Turkey; [Korkmaz] Halil, Department of Chemistry, Ondokuz Mayis Üniversitesi, Samsun, Turkeyen_US
dc.description.abstractThe kinetics of oxidation of rainbow trout (Oncorhynchus mykiss) NADPH-cytochrome P450 reductase (NCPR) by cytochrome-c and NADH-ferricyanide reductase (NFR) by K<inf>3</inf>Fe(CN)<inf>6</inf> were studied. NCPR and NFR activities from liver microsomes of rainbow trout (Oncorhynchus mykiss) had a specific activity of 0.0246 and 0.0350 nmoles/min/mg protein, respectively. The maximal rate of NCPR reaction was found at cytochrome-c concentrations above 2.1 mM and in the presence of cyt c. V<inf>max</inf> and K<inf>m</inf> were 0.0083 nmoles/min/mg and 1.14 mM. The maximal rate of NFR reaction was at K<inf>3</inf>Fe(CN)<inf>6</inf> concentrations higher than 2.0 mM and in the presence of K<inf>3</inf>Fe(CN)<inf>6</inf>. V<inf>max</inf> and K<inf>m</inf> values were 1.04 nmoles/min/mg and 0.352 mM, respectively. The inhibition by dichlorvos (DDVP) on NCPR and NFR activity was not exhibited over the range 1.0-5.0 mM DDVP.en_US
dc.identifier.endpage44en_US
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-1842731808
dc.identifier.startpage35en_US
dc.identifier.volume56en_US
dc.identifier.wosWOS:000220788300007
dc.language.isoenen_US
dc.publisherIsraeli Journal of Aquaculture-Bamidgehen_US
dc.relation.ispartofIsraeli Journal of Aquaculture - Bamidgehen_US
dc.relation.journalIsraeli Journal of Aquaculture-Bamidgehen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDDVPen_US
dc.subjectMicrosomesen_US
dc.subjectNADH-Ferricyanide Reductaseen_US
dc.subjectNADPH-Cytochrome P450 Reductaseen_US
dc.subjectOncorhynchus Mykissen_US
dc.titleEffects of Dichlorvos and Characterization of Microsomal NADPH-Cytochrome P450 and NADH-Ferricyanide Reductase in Rainbow Trout (Oncorhynchus Mykiss) Liveren_US
dc.typeArticleen_US
dspace.entity.typePublication

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