Nonomuraea Muscovyensis sp. Nov., Isolated from Soil

Abstract

A novel actinomycete, strain FMN03T, was isolated from a soil sample collected from Yuga Zapadnaya South-West Forest Park, Moscow, Russia. The isolate had chemical and morphological properties typical of members of the genus Nonomuraea and formed a distinct 16S rRNA gene subclade with the type strains Nonomuraea roseoviolacea subsp. carminata NBRC 15903T and Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098T. The organism formed extensively branched substrate and aerial hyphae, which generated spiral chains of spores with smooth surfaces. The cell wall contained meso-diaminopimelic acid and the whole cell sugars were glucose, galactose and trace amounts of madurose, mannose and xylose. The polar lipids were phosphatidylethanolamine, hydroxyphosphatidylethanolamine, four unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. The predominant menaquinone was MK-9(H<inf>4</inf>). The major fatty acids were iso-C<inf>16: 0</inf> 2-OH, C<inf>17: 0</inf> 10-methyl, C<inf>17: 1</inf> cis9 and iso-C<inf>16: 0</inf>. Analyses of its morphological, physiological and biochemical characteristics, together with DNA-DNA relatedness data, confirmed that strain FMN03T is a representative of a novel species of the genus Nonomuraea, which is distinct from closely related reference strains. Strain FMN03T (= DSM 45913T = KCTC 29233T) is proposed as the type strain of a novel species, for which the name Nonomuraea muscovyensis sp. nov. is proposed. © 2014 IUMS.