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Use of Rep-PCR for Genetic Diversity Analyses in Fusarium culmorum

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dc.authorscopusid6507049596
dc.authorscopusid6505804541
dc.authorscopusid57204374030
dc.authorscopusid35738507800
dc.authorscopusid22137134400
dc.contributor.authorGürel, F.
dc.contributor.authorAlbayrak, G.
dc.contributor.authorDiken, O.
dc.contributor.authorÇepni, E.
dc.contributor.authorTunali, B.
dc.date.accessioned2020-06-21T14:48:33Z
dc.date.available2020-06-21T14:48:33Z
dc.date.issued2010
dc.departmentOndokuz Mayıs Üniversitesien_US
dc.department-temp[Gürel] Filiz, Department of Molecular Biology and Genetics, Istanbul Üniversitesi, Istanbul, Turkey; [Albayrak] Gülruh, Department of Molecular Biology and Genetics, Istanbul Üniversitesi, Istanbul, Turkey; [Diken] Ozlem, Department of Molecular Biology and Genetics, Istanbul Üniversitesi, Istanbul, Turkey; [Çepni] Elif, Department of Molecular Biology and Genetics, Istanbul Üniversitesi, Istanbul, Turkey; [Tunali] Berna, Department of Plant Protection, Ondokuz Mayis Üniversitesi, Samsun, Turkeyen_US
dc.description.abstractFusarium culmorum is a pathogen of economically important grain crops. In this work, Rep-PCR was used to identify genetic diversity in F. culmorum isolates which have been collected from wheat fields in Turkey. Reproducible genomic fingerprints were amplified in each strain by PCRs of prokaryotic repetitive extragenic palindromic (REP), enterobacterial repetitive intergenic consensus (ERIC) and BOX sequences. Totally 104 molecular markers were evaluated and similarity comparisons were shown as a dendrogram. The average genetic diversity was 52.3% ranging from 15.8% to 88.7% according to the Rep-PCR data. Cluster analysis showed agreement with the distance of sampling locations. The highest genetic similarity (84.2%) was determined between two F. culmorum isolates (F1 and F2) originated from the same agro-ecological region. Our results showed that Rep-PCR is convenient and rapid for genetic diversity analyses and strain differentiation in F. culmorum. © 2009 Blackwell Verlag GmbH.en_US
dc.identifier.doi10.1111/j.1439-0434.2009.01630.x
dc.identifier.endpage389en_US
dc.identifier.issn0931-1785
dc.identifier.issn1439-0434
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-77953097285
dc.identifier.scopusqualityQ4
dc.identifier.startpage387en_US
dc.identifier.urihttps://doi.org/10.1111/j.1439-0434.2009.01630.x
dc.identifier.volume158en_US
dc.identifier.wosWOS:000276491700010
dc.identifier.wosqualityQ3
dc.language.isoenen_US
dc.publisherWiley-Blackwell Publishing, Incen_US
dc.relation.ispartofJournal of Phytopathologyen_US
dc.relation.journalJournal of Phytopathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFusarium Culmorumen_US
dc.subjectGenetic Diversityen_US
dc.subjectREP-PCRen_US
dc.titleUse of Rep-PCR for Genetic Diversity Analyses in Fusarium culmorumen_US
dc.typeArticleen_US
dspace.entity.typePublication

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