Selective isolation and molecular identification of different actinomycetes from various habitats

Abstract

Isolation of actinomycetes is still valuable from different habitats because these organisms are vital groups for clinical, ecological and agricultural applications. This study was aimed to identify the possible novel actinomycetes that are potentially secondary metabolite producers, and to gain novel species for literature. In this study, different soil samples were used for isolation of different actinomycete strains, and totally 2169 isolates were obtained with selective and conventional isolation methods. The isolates are selected according to the results of chemotaxonomy tests and various morphological characteristics for further molecular tests. The PCR-based molecular method, which is the commonly used in identification of bacterial isolates and in determination of phylogenetic relationships, was used for obtaining 16S rRNA gene sequences. A total of 107 isolates, 41 of which contain mycolic acids, were analysed in terms of 16S rRNA gene sequences. Taxonomic positions of actinomycete isolates were determined by analysing 16S rRNA data sets. Accession numbers from NCBIand culture collection numbers from the culture collections (DSMZ and KCTC) were provided for the possible novel actinomycete isolates of this study.