The effects of Pseudomonas putida biotype B on Tetranychus urticae (Acari: Tetranychidae) [2]

Abstract

This study investigated Pseudomonas putida biotype B as a potential biological control agent of Tetranychus urticae. The bacteria were isolated from greenhouse soil from Carsamba, Turkey. The experiment was carried out in a completely randomized plot design under laboratory conditions. For this purpose, spraying and dipping applications of a suspension of P. putida biotype B (108-109 colony forming units/ml) were applied to newly emerged, copulated females. Dead mite and egg counts were started on the 3rd day after treatments, and observations were continued daily until all the mites had died and egg hatching had finished. Both types of bacterial application significantly reduced total egg numbers and egg hatching, compared to their respective controls. Bacterial spraying was significantly more effective than dipping - the spray application demonstrated 100% efficacy and resulted in the fewest viable eggs. The results of this study indicated that P. putida biotype B has a strong efficacy in causing mortality in T. urticae. © Springer Science+Business Media B.V. 2009.