The prevalence of seven crucial honeybee viruses using multiplex RT-PCR and their phylogenetic analysis
MetadataShow full item record
CitationCAGIRGAN A. A,YAZICI Z (2021). The prevalence of seven crucial honeybee viruses using multiplex RT-PCR and their phylogenetic analysis. Turkish Journal of Veterinary and Animal Sciences, 45(1), 44 - 55. Doi: 10.3906/vet-2004-139.
Honey bee viruses may be of severe adverse effect on bee colonies. Till now, more than twenty honey bee viruses have been identified. The aim of this study was to investigate the prevalence of seven honey bee viruses, namely Israeli acute paralysis virus (IAPV), deformed wing virus (DWV), sacbrood virus (SBV), acute bee paralysis virus (ABPV), black queen cell virus (BQCV), Kashmir bee virus (KBV), and chronic bee paralysis virus (CBPV) using a multiplex reverse transcription polymerase chain reaction (mRT-PCR). A total of 111 apiaries were randomly selected and adult bees and larvae samples were obtained from seemingly healthy colonies located at Aegean region of west Turkey. The presence of black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), acute bee paralysis virus (ABPV), and sacbrood virus (SBV) was detected, while Israeli acute paralysis virus (IAPV) or Kashmir bee virus (KBV) couldn’t be detected in studied colonies. The results showed the virus with the highest prevalence was DWV followed by BQCV, ABPV, SBV, and CBPV. PCR products for DWV, BQCV, ABPV, SBV, CBPV were sequenced and compared to the Genbank database. The Turkish strains demonstrated heterogeneous populations of DWV, BQCV, and relatively homogenous populations of ABPV, CBPV, and SBV. Based on these results, SBV and CBPV had formed different branch and genotypes when compared to previous studies. In conclusion, this study provides information about the distinctive honey bee viruses for future researches in Turkey, which holds the largest number of managed colonies in Europe. Key words: Apis mellifera, deformed wing virus, black quenn cell virus, sacbrood virus, chronic bee paralysis virus, acute bee paralysis virus
SourceTurkish Journal of Veterinary and Animal Sciences
Showing items related by title, author, creator and subject.
Samsun ilinde Beet necrotic yellow vein virus ve/veya Beet soilborne virus ile bulaşık şeker pancarı üretim alanlarında virüs taşıyan Polymyxa betae Keskin oranının belirlenmesi Bu çalışma, Samsun ili şeker pancarı (Beta vulgaris L.) üretim alanlarına ait toprak örneklerinde „En Yüksek Olası Sayı? (Most probable number: MPN) tekniği ile Beet necrotic yellow vein virus (BNYVV) ve/veya Beet soilborne ...
Samsun ilinde fasülye alanlarında enfeksiyon oluşturan Bean common mosais virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV)'un ırklarının belirlenmesi ve bazı fasülye çeşitlerinin BCMV ve BCMNV'ye karşı dayanıklılık düzeylerinin araştırılması / İlyas Deligöz; Danışma Miray Sökmen Deligöz, İlyas (Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, 2007)Samsun ilinde fasulye (Phaseolus vulgaris L.) yetiştiriciliğinin yapıldığı alanlardan alınan ve Bean common mosaic virus (BCMV) ve Bean common mosaic necrosis virus (BCMNV) ile enfekteli olduğu belirlenen örneklere ait ...