P16 staining patterns and their relationships with clinical parameters in cases of cervical intraepithelial neoplasia

Abstract

The aim of this study is to investigate the contribution of the p16 immunohistochemical marker in the cervical intraepithelial neoplasia (CIN) cases in the distinctionbetween CIN1, CIN2, CIN3 and the relationship of CINs with age, localization. CIN1, CIN2, CIN3 preparations of 2015 in the pathology department were retrospectivelyanalyzed. New sections of 3 μm thickness were obtained on polylysine slides from paraffin blocks of these tissues. Samples with immunohistochemical staining with p16were evaluated under a light microscope.p16 expression was semiquantitatively assessed according to staining intensity with 4 staining levels as score 0,1,2,3. As a resultof the Kruskal-Wallis test, a significant difference was found between the scores in the CIN groups (p <0,001). As a result of the multiple comparison test, p16 scoresof patients in the CIN2 and CIN3 group were found to be significantly higher than in the CIN1 group (p <0.001). While the mean age of patients in the CIN3 group wassignificantly higher than those in the CIN2 group (p <0.05), it was not different from the patients in the CIN1 group (p> 0.05). The variations between CIN groups andanatomical locations on the cervix were examined with Chi-square test. There was no change in the frequency distribution of CIN groups in the anatomical locations onthe cervix (p> 0.05). In cervical intraepithelial neoplasms, p16 was found to show increased staining with the severity of the lesion. It was observed that p16 expression isimportant in CIN evaluation and reduces