The comparison of gonadotropin-releasing hormone agonist therapy and intraperitoneal Ringer's lactate solution in prevention of postoperative adhesion formation in rat models

Abstract

Objective: To evaluate gonadotropin-releasing hormone agonists (GnRH-a) therapy and intraperitoneal Ringer's lactate solution (RL) for the prevention of postsurgical adhesion formation in rat models. Study Design: Forty rats were randomized into four groups: Group I was the control; in group II, RL was applied at the surgery; group III and IV received preoperative subcutaneous injections of the GNRH-a leuprolide acetate and only the diluent of the GNRH-a respectively. A standard lesion was created over the uterus of each rat. Adhesions were scored at relaparotomy 3 weeks later. Analysis of variance (Kruskal-Wallis) and Mann-Whitney U-tests were used fur the statistical evaluation. Results: The average adhesion scores were 2.7 +/- 0.26, 1.4 +/- 0.27, 0.9 +/- 0.18, and 2.5 +/- 0.34 in groups I, II, III, and IV respectively. There were significant differences between the scores of groups I and II (P < 0.01), groups I and III (P < 0.001), groups II and IV (P < 0.05), and groups III and IV (P < 0.01); but not between the scores of groups II and III, and groups I and IV (P > 0.05). Conclusion: GNRH-a was successful in reducing postoperative adhesion formation but was not superior to intraperitoneal RL. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.