Spectrophotometric flow-injection analysis of mercury(II) in pharmaceuticals with p-nitrobenzoxosulfamate
Özet
A new, simple and rapid spectrophotometric FI method for the accurate band precise determination of Hg(II) in pharmaceutical preparations has been developed. The method is based on the measuring the decrease of absorbance intensity of p-nitrobenzoxosulfamate (NBS) due to the complexation with Hg(II). The absorption peak of the NBS, which is decreased linearly by addition of Hg(II), occurs at 430 nm in 2 x 10(-4) mol l(-1) HNO3 as a carrier solution. Optimization of chemical and FI variables has been made. A micro column consisting of several packing materials applied instead of reaction coil was also investigated. A background level of Fe(III) maintained in reagent carrier solution with NBS was found useful for sensitivity and selectivity. Under the optimized conditions, the sampling rate was over 100 h(-1), the calibration curve obtained were linear over the range 1-10 mug ml(-1), the detection limit was lower than 0.2 mug ml(-1) for a 20 mul injection volume, and the precision [S-r = 1% at 2 mug ml(-1) Hg(II) (n = 10)] was found quite satisfactory. Application of the method to the analysis of Hg(II) in pharmaceutical preparations resulted a good agreement between the expected and found values. (C) 2003 Elsevier Science B.V. All rights reserved.
