The Investigation of Obesity Susceptibility with IL-4 Gene Intron 3 VNTR and IL-6 Gene-597G/A Polymorphisms in a Turkish Population

Abstract

Obesity results in a proinflammatory state starting within the metabolic cells as adipocyte, monocyte. The bioactive system, including cytokines as IL-4 and IL-6, contributes to the pathogenesis of conditions associated with obesity and favors inflammation. This study aimed to investigate the role of IL-4 gene intron 3 VNTR and IL-6 gene 597G/A polymorphisms in the pathogenesis of obesity. The study included 127 patients with obesity (BMI>30kg/m(2)) and 110 healthy controls. Genomic DNA was isolated and genotyped using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis for IL-4 gene and IL-6 gene polymorphisms. There was no statistical significant difference in the allele and genotype frequencies of both genes polymorphism in obesity patients and control groups (p>0.05). Additionally, there was no statistical significant difference in the combine genotype analysis of IL-4 gene intron 3 VNTR and IL-6 gene -597G/A polymorphism (p>0.05). These findings showed that there are not any associations of IL-4 gene intron 3 VNTR and IL-6 gene -597G/A polymorphisms with susceptibility of a person for development of obesity. This is the first report that investigates the relationships between susceptibility of obesity and IL-4gene intron 3 VNTR, IL-6 gene -597G/A polymorphisms.