Evaluation of suppression of rhizomania disease by earthworm (Lumbricus terrestris L.) and its effects on soil microbial activity in different sugar beet cultivars

Abstract

The ability of earthworm Lumbricus terrestris L. to suppress the multiplication of Beet necrotic yellow vein virus (BNYVV) transmitted by Polymyxa betae and its effects on soil microbial activity were investigated under controlled conditions. BNYVV-infested and a combination of earthworm with BNYVV-infested soil were compared to non-infested soils for their effects on plant and root weights and virus content of two different sugar beet cultivars by using partially resistant (cv. Leila) and susceptible (cv. Arosa) cultivars to the rhizomania disease. Soil testing with sugar beet baiting plants followed by enzyme-linked immunosorbent assay (ELISA) was used to diagnose virus. The results of the statistical analyses showed that total fresh plant and root weights were negatively correlated with BNYVV infection. Addition of L. terrestris significantly enhanced plant and root weights. The earthworm-added soils had higher microbial activity such as basal soil respiration and dehydrogenase activity. The presence of earthworms in the soil did not statistically suppress BNYVV infection (p< 0.05). Sugar beet production may be enhanced by using resistant cultivars with adding L. terrestris into soil where rhizomania is present.