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dc.contributor.authorKarami, Kazem
dc.contributor.authorRahimi, Mahzad
dc.contributor.authorZakariazadeh, Mostafa
dc.contributor.authorBüyükgüngör, Orhan
dc.contributor.authorAmirghofran, Zahra
dc.date.accessioned2020-06-21T13:05:38Z
dc.date.available2020-06-21T13:05:38Z
dc.date.issued2018
dc.identifier.issn0022-328X
dc.identifier.issn1872-8561
dc.identifier.urihttps://doi.org/10.1016/j.jorganchem.2018.09.018
dc.identifier.urihttps://hdl.handle.net/20.500.12712/11232
dc.descriptionWOS: 000449711200007en_US
dc.description.abstractThe ylide-phosphonium salt [PPh3CH2C(O)CH2Cl]Cl-+(-) was reacted with Pd(OAc)(2) to give the chlorobridged dinuclear complex [Pd{C(H)PPh3C(O)CH2Cl}(mu-Cl)(OAc)](2), which experienced bridge cleavage reactions with triphenylphosphine (PPh3) and pyridine (Py), and to prepare the new orthometallated complexes [Pd{(C,C)-C6H4 PPh2C(H)C(O)CH2Cl}L]Cl, [L = PPh3 (1) and Py (2)]. The complexes were identified and characterized using various techniques. X-ray crystallography was used to determine the crystal structure of 1, which revealed the presence of an orthometallated C6H4-PPh2 unit. CT-DNA binding interaction of the synthesis compounds was tested by fluorescence spectroscopy, UV-Vis absorption spectroscopy, and the viscometric titration method. The analysis of the obtained data indicated that the Pd complexes could bind to DNA via groove binding by the partial intercalation mode. The emission titration of bovine serum albumin (BSA) with two Pd complexes showed a static process for the fluorescence quenching mechanism of BSA. In addition, the results of competitive binding by Eosin-Y, Ibuprofen and Digoxin site markers revealed that the complexes were bound to the site I of BSA. The donor (BSA) - acceptor (Pd complexes) distance was calculated using fluorescence resonance energy transfer (FRET). Notably, molecular docking studies were used for the determination of DNA and BSA-Pd (II) complexes binding. Finally, the two complexes exhibited significant in vitro cytotoxicity against human leukemic T cell (Jurkat) and chronic myelogenous leukemia (K562) cancer cell lines using MTT([3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] colorimetric. In cell cycle analysis conducted on Jurkat and K562 cells treated with ligand and Pd complexes, a decrease in DNA cell content and shift in the main population of cells toward the subGl phase were observed. (C) 2018 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipIsfahan University of Technologyen_US
dc.description.sponsorshipThis research was funded by Isfahan University of Technology. Crystallography was provided by the Faculty of Arts and Sciences, Department of Physics, Ondokuz Mayrs University.en_US
dc.language.isoengen_US
dc.publisherElsevier Science Saen_US
dc.relation.isversionof10.1016/j.jorganchem.2018.09.018en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOrthometallated complexesen_US
dc.subjectBSA bindingen_US
dc.subjectCT-DNA bindingen_US
dc.subjectAnticancer activityen_US
dc.subjectMolecular dockingen_US
dc.titleNew phosphorus ylide palladacyclic: Synthesis, characterization, X-Ray crystal structure, biomolecular interaction studies, molecular docking and in vitro cytotoxicity evaluationsen_US
dc.typearticleen_US
dc.contributor.departmentOMÜen_US
dc.identifier.volume878en_US
dc.identifier.startpage60en_US
dc.identifier.endpage76en_US
dc.relation.journalJournal of Organometallic Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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