Determination of extended spectrum beta-lactamase producing Enterobacteriaceae from cheese samples sold in public bazaars

Abstract

In this study, it was aimed to determinate the prevalence of extended spectrum beta-lactamases (ESBL) producing Enterobacteriaceae and genes responsible for resistance formation in cheeses that were sold in local markets in Samsun. A total of 150 cheese samples were collected from the local markets in Samsun center and were used as material. One hundred and forty-eight ESBL positive isolates were obtained from 34 samples (26.6%). Identification of 148 isolates were as follows; 79 Escherichia coli (54.5%), 39 Klebsiella pneumoniae (26.3%), 16 Klebsiella oxytoca (10.8%), 5 Citrobacter youngae (3.4%), 4 Shigella bodii (2.7%), 2 Klebsiella ozaenae (1.53%), 2 Enterobacter cloacae (1.53%), and 1 Enterobacter aerogenes (0.67%). Polymerase chain reaction analysis indicated that 64 isolates carried the bla(CTX-M) gene (43.2%), while 39 isolates carried the bla(TEM) gene (26.3%), and 16 isolates carried the bla(SHV) gene (10.8%). It was concluded that cheeses sold in local markets are an important source of enteric bacteria that can produce ESBL. Therefore, cheeses can be considered as a potential source for spread of ESBL genes from foods to human. Practical Applications Enterobacteriaceae strains producing ESBL have been isolated from many foods, especially foods of animal origin, form a potential risk to public health due to zoonotic character.